| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249453 |
| Chromosome: | chromosome 6 |
| Location: | 4881833 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279000 | PBA1 | (1 of 2) K02738 - 20S proteasome subunit beta 1 (PSMB6); 20S proteasome beta subunit A1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCACTGCACGGTCCTTGTGCTAGTTGC |
| Internal bar code: | GCTTGACAGTCGACGGTCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 99.4907 |
| LEAP-Seq n confirming: | 1172 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCCTGCCCATACAGAGC |
| Suggested primer 2: | CATCAATCAACACAGACGCC |