| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249525 |
| Chromosome: | chromosome 1 |
| Location: | 3987520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025950 | PPR8 | (1 of 1) IPR000209//IPR002885 - Peptidase S8/S53 domain // Pentatricopeptide repeat; PentatricoPeptide Repeat protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAGCTAGCGCCTTTGCGCCTCTTCCAC |
| Internal bar code: | GCCCCGCACAGGTAGGGCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 93.2907 |
| LEAP-Seq n confirming: | 1460 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTAGGGGTGTCCGTGAA |
| Suggested primer 2: | AACGGCAGCTGCTTAGTGAT |