| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249547 |
| Chromosome: | chromosome 13 |
| Location: | 1684098 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574150 | FAO10 | FAD-dependent oxidoreductase; (1 of 1) 1.1.99.2 - L-2-hydroxyglutarate dehydrogenase / L-alpha-hydroxyglutarate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCCCATCCAGTGTCAACAATTCCATC |
| Internal bar code: | CAATCTGGGTACGGCTCATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 99.786 |
| LEAP-Seq n confirming: | 1399 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCAAGAGGTGGCGTTTC |
| Suggested primer 2: | CTGTCGTTTCACTTCAGCCA |