| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249623 |
| Chromosome: | chromosome 2 |
| Location: | 3538711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095127 | (1 of 3) PTHR11732//PTHR11732:SF146 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCATGGAATGAGGAGGTGGGTGAACCGT |
| Internal bar code: | TAGGTTATCGTCGCCTAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 98.5507 |
| LEAP-Seq n confirming: | 1020 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCATTTGTGGCTGTAGAT |
| Suggested primer 2: | TGTCCTTACTTGCGCTGATG |