Insertion junction: LMJ.RY0402.249668_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249668
Chromosome:chromosome 8
Location:4746913
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g384250 SNRK2O (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATCGGAAGCAAATTTGATATACATCAG
Internal bar code:GCGCAGGTCGAGTACTGATCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:99.019
LEAP-Seq n confirming:1514
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTGCAGGTGTAGCGATG
Suggested primer 2:CAACACACAACCTGGCAATC