| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249680 |
| Chromosome: | chromosome 6 |
| Location: | 7405165 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299600 | ZRT6,ZRT4,ZIP6 | Zinc-nutrition responsive transporter; (1 of 1) PTHR11040:SF57 - PROTEIN F31C3.4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGAGCAGCAGCTGCAGGAGCGACAGGA |
| Internal bar code: | TAATATGTTGTTCTACCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 95.9459 |
| LEAP-Seq n confirming: | 3053 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGATTTCCAACTCCAATG |
| Suggested primer 2: | GGTGCCATGGAGCAAGTTAT |