| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249701 |
| Chromosome: | chromosome 12 |
| Location: | 9383300 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540200 | HEL58 | (1 of 5) K12823 - ATP-dependent RNA helicase DDX5/DBP2 (DDX5, DBP2); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTAATCCTCGCCGCCCACGTGCCCCC |
| Internal bar code: | GCAGGAGAAGGTTCGATATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 201 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCAACTACGACTTCCCC |
| Suggested primer 2: | GCTTGGCCATCTTCTTTCTG |