| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249712 |
| Chromosome: | chromosome 3 |
| Location: | 4994706 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180300 | SIR3 | (1 of 1) 1.8.1.2 - Assimilatory sulfite reductase (NADPH) / Sulfite reductase (NADPH); Sulfite reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTTCACCTTGACCAACATGCGCACAA |
| Internal bar code: | GGACACAGCAGAGTCCGATCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 98.7448 |
| LEAP-Seq n confirming: | 236 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACGTTGGGAGACTGAAC |
| Suggested primer 2: | AAGGTGTACGTCCAGAACCG |