| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249791 |
| Chromosome: | chromosome 1 |
| Location: | 3363280 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021251 | ASL,ARG7 | Argininosuccinate lyase; (1 of 1) 4.3.2.1 - Argininosuccinate lyase / Omega-N-(L-arginino)succinate arginine-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGCCTCGAATTGCATTGCAGCAGCAGA |
| Internal bar code: | AAGGCGGAAGGGACTGTGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 99.5064 |
| LEAP-Seq n confirming: | 1008 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCAGAAACCAGAAGCAC |
| Suggested primer 2: | CCGGGATGGATTAAGGATCT |