| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249828 |
| Chromosome: | chromosome 8 |
| Location: | 873076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361250 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTGGAGTCAGCAGTACCCCTCCCTCC |
| Internal bar code: | CGAAGGGCCGTCTGTAGACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 98.855 |
| LEAP-Seq n confirming: | 1295 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTAAGTTTGGGCTGG |
| Suggested primer 2: | AATGGGCGTGTCGATAAAAG |