| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249829 |
| Chromosome: | chromosome_17 |
| Location: | 2405147 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g715300 | PKD2 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCCTTCAGCAAAGCTGCAGCGCGGCTTC |
| Internal bar code: | CCGGACATCGAAGTTGCTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 99.0408 |
| LEAP-Seq n confirming: | 2065 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATGGTTCGTAGCGTTT |
| Suggested primer 2: | CGAATCTGCTATACGACGCA |