| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.249849 |
| Chromosome: | chromosome 2 |
| Location: | 7658898 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143250 | IDH2 | (1 of 2) 1.1.1.41 - Isocitrate dehydrogenase (NAD(+)) / Nicotinamide adenine dinucleotide isocitrate dehydrogenase; Isocitrate dehydrogenase, NAD-dependent | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCCGCACTGCCTCCTCTACCCACTCAC |
| Internal bar code: | TGTTGGGTTGACGGCCCGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 99.9161 |
| LEAP-Seq n confirming: | 1191 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTTAACATGGGGAGGCG |
| Suggested primer 2: | AGTTGGTGGTGAGGGATGAG |