| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249849 |
| Chromosome: | chromosome_2 |
| Location: | 7658909 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g143250 | IDH2 | Isocitrate dehydrogenase, NAD-dependent | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAATGCACGATGGTGAGCTCTGAGCAGCT |
| Internal bar code: | GTACGGCGGGATGTATGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTTAACATGGGGAGGCG |
| Suggested primer 2: | AGTTGGTGGTGAGGGATGAG |