| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.249871 |
| Chromosome: | chromosome 11 |
| Location: | 1160549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467687 | (1 of 1) 1.1.1.348 - Vestitone reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGACACCCCGTGCGACCGCTTCTCTC |
| Internal bar code: | CCCATTGCCTGGCATGTCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 99.7093 |
| LEAP-Seq n confirming: | 1029 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTCACAAGCCTTCTCGT |
| Suggested primer 2: | GAGGAGTTGAGGCTGTCTGG |