| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249892 |
| Chromosome: | chromosome 13 |
| Location: | 3202900 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585600 | (1 of 1) IPR011333//IPR019832 - POZ domain // Manganese/iron superoxide dismutase, C-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCAGGTGCAGATTAGCTGCACGTCGAG |
| Internal bar code: | CCTAGAGCCGTGTTTTCACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 600 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGGCCTTGCTGTGAAA |
| Suggested primer 2: | CTACGTAAGGCCCTCAGCAC |