| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249930 |
| Chromosome: | chromosome 4 |
| Location: | 359184 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217911 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCTGGAAGGGACAAGGAAGCAGCAGG |
| Internal bar code: | ATAAAATCGGTGGTTTGCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 93.4066 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACGTGGAGCTTAGGAGC |
| Suggested primer 2: | AACACCTACCATGAGGCACC |