| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249985 |
| Chromosome: | chromosome 1 |
| Location: | 4321220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029200 | DIV30,ESP1 | Separase, cell cycle protease; (1 of 1) 3.4.22.49 - Separase / Separin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTTGATGCTCGGATAGCAGCACTCTGT |
| Internal bar code: | TGGGGACGATAAAAAGATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.6212 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTATGCTCTCAAACGCACG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |