Insertion junction: LMJ.RY0402.249993_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249993
Chromosome:chromosome 17
Location:1290977
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g705650 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGAGGCAGCAAAAGGCCCCTGTCGACCC
Internal bar code:CGTATCGGCCGATTTGGCGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:375
LEAP-Seq percent confirming:95.7143
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTCATGTCGATAGCAC
Suggested primer 2:CACCTCCCTCCATTCCTGTA