Insertion junction: LMJ.RY0402.250014_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.250014
Chromosome:chromosome 6
Location:2501106
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g269000 (1 of 1) PF09493 - Tryptophan-rich protein (DUF2389) (DUF2389) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATATCTTTCTGCAGGTGTTGCGCAAACAA
Internal bar code:CGCAACATCAAGGACGCCATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:429
LEAP-Seq percent confirming:98.9247
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCGCTCCTATACCTCGC
Suggested primer 2:TACTCGGCTGATTTGCAGTG