| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250085 |
| Chromosome: | chromosome 3 |
| Location: | 8010171 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201550 | MMP20 | Matrix metalloproteinase; (1 of 1) IPR000095//IPR008752 - CRIB domain // Peptidase M11, gametolysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCACGGGGCCGGCGCTGCCCATTGGATC |
| Internal bar code: | ACTCTCGTCCGTTTCTGTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.6723 |
| LEAP-Seq n confirming: | 13383 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTGTGCATGTGATGA |
| Suggested primer 2: | CAGGTCTAGCATGGTGAGCA |