| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250087 |
| Chromosome: | chromosome 9 |
| Location: | 7115012 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411850 | (1 of 2) PTHR23130:SF61 - DEFENSE PROTEIN L(2)34FC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGAGGGAGCTCTTCTTGGTAAGTCTA |
| Internal bar code: | ACAGAGAGGGCACGCTTGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1157 |
| LEAP-Seq percent confirming: | 98.6612 |
| LEAP-Seq n confirming: | 1695 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTCAGTATTGACAGGT |
| Suggested primer 2: | TATGCGTGCCTCTCGTACTG |