| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250103 |
| Chromosome: | chromosome_2 |
| Location: | 2227486 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g089950 | IFT20 | Intraflagellar transport protein 20 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGATACTGTAGCGTCGCTTCTGCACGAC |
| Internal bar code: | TTATCGATAGCTCGAAGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 863 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTGACAGGAGCGGAAAC |
| Suggested primer 2: | CTTCAACCTTCTCCGCACTC |