| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250104 |
| Chromosome: | chromosome 17 |
| Location: | 5987882 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740550 | GT90-33,GT90F33 | GT90 family protein 33; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAGACGTCCTGCCTCCCGGTGCCTCTG |
| Internal bar code: | GCGCCAAGATCGCGAGTGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1121 |
| LEAP-Seq percent confirming: | 67.0886 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGAGCACTGTAACGCCA |
| Suggested primer 2: | CGTGAACCACAAATCTCCCT |