| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250132 |
| Chromosome: | chromosome 9 |
| Location: | 6790491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409500 | RPP25 | Ribonuclease MRP subunit P25; (1 of 2) PF01918 - Alba (Alba) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAAGGGCACGCATCCGCCGGTCGGCTT |
| Internal bar code: | TCGCTGGGCACCTTCAAGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCACAAGAACTCAACGAG |
| Suggested primer 2: | GCTAGCGTAGCAACTTTGGG |