| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250136 |
| Chromosome: | chromosome 12 |
| Location: | 83401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483800 | CPY | Serine carboxypeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGATGTAATGAATACAGCATCCGCGGCC |
| Internal bar code: | TCGGCAGGTCGTAGAGACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 93.4363 |
| LEAP-Seq n confirming: | 242 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACCGTACCTCCTTTCC |
| Suggested primer 2: | TGCCCTCATAAATCCTACGC |