| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250139 |
| Chromosome: | chromosome 17 |
| Location: | 5356456 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736474 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATTCATGCAGGGTACACCCGCAGCGGA |
| Internal bar code: | CCGGGTGATGGTCCCTGCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 99.4824 |
| LEAP-Seq n confirming: | 961 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGACATGGTTCGTACAC |
| Suggested primer 2: | GCGAAGTGTGTGGTGTGACT |