| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250215 |
| Chromosome: | chromosome 9 |
| Location: | 6504724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407501 | (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCACAGGCGACTTCTTCTCCACACATG |
| Internal bar code: | CGGGACCGTGCACTCAGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 98.5047 |
| LEAP-Seq n confirming: | 1054 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCACATGAGTTAGCAA |
| Suggested primer 2: | CTGTTGACTCCTGGGGATGT |