| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250232 |
| Chromosome: | chromosome 12 |
| Location: | 7279704 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557750 | DGTT1 | Diacylglycerol O-acyltransferase, Type 2; (1 of 2) PTHR12317:SF0 - DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCTTGTGCTGTGGCATATGTATTATAT |
| Internal bar code: | GTCCGTCGCAGTCCATAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 99.7191 |
| LEAP-Seq n confirming: | 355 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGTACGGCAAGGGTGTC |
| Suggested primer 2: | GGACCAAGTACTGAGCAGGC |