| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250259 |
| Chromosome: | chromosome 3 |
| Location: | 5361583 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184350 | (1 of 1) K13094 - RNA-binding protein 5/10 (RBM5_10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGTATTTGACAAGACGAGAGGCGGTC |
| Internal bar code: | TCCGGCATGACCTCCCCTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCGTTCCTCAATCTCTT |
| Suggested primer 2: | TGTGTGCATGAGTGTAGCCA |