Insertion junction: LMJ.RY0402.250272_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.250272
Chromosome:chromosome 16
Location:961049
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g648750 (1 of 1) PF10373 - Est1 DNA/RNA binding domain (EST1_DNA_bind) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGTTACCCAGCACATCATGCATGGCCCA
Internal bar code:CAGCTAGCGACGGTAGGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:91.4468
LEAP-Seq n confirming:1005
LEAP-Seq n nonconfirming:94
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AAAACACGCCCACATACACA
Suggested primer 2:GTCACCACACCGCATACAAC