| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250324 |
| Chromosome: | chromosome 10 |
| Location: | 4331304 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCGTAGCCATCCACTCGGTTAGACCAC |
| Internal bar code: | AGACTGGGGGATCAAGAGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 92.9487 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGGTGATTATGCAGGA |
| Suggested primer 2: | ATGCTGAGAGGTGAGCAGGT |