| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250380 |
| Chromosome: | chromosome 1 |
| Location: | 7268379 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCCCGCCCACGCTCACACGCTCCACCT |
| Internal bar code: | TGGCATTAGGCTTTAGGAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 98.2344 |
| LEAP-Seq n confirming: | 612 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTTCCTGCCTCCCTAAG |
| Suggested primer 2: | TATCCCAGGTATGCTGCCTC |