Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.250386 |
Chromosome: | chromosome 16 |
Location: | 1860231 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g655550 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTGGAATGGGGGCGGTACGACTGGTA |
Internal bar code: | CGGAGCGACCATATGGAACGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 182 |
LEAP-Seq percent confirming: | 96.6967 |
LEAP-Seq n confirming: | 322 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGTTTCCAAACTCCTCACC |
Suggested primer 2: | CTCCTCCTCCTCCTGTTGCT |