Insertion junction: LMJ.RY0402.250390_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.250390
Chromosome:chromosome 6
Location:5620347
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g285900 FAP396 (1 of 2) IPR000104//IPR003590 - Antifreeze protein, type I // Leucine-rich repeat, ribonuclease inhibitor subtype; Flagellar Associated Protein 396 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAAGAGGTTGACGGAGCTGACGGACGTG
Internal bar code:TGTTTTCGTCTCAAATGCGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:126
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGTGAGCTTGGACCATT
Suggested primer 2:CTCTCAGCTGGGCAAGTACC