Insertion junction: LMJ.RY0402.250415_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.250415
Chromosome:chromosome 16
Location:6283065
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g674700 AGH1 ADP-ribosyl glycohydrolase; (1 of 1) 3.2.2.24 - ADP-ribosyl-[dinitrogen reductase] hydrolase / Dinitrogenase reductase activating glycohydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCATTCATAGCCGGCACGCCACAAACC
Internal bar code:CATACGACATGTTCCCACGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAGGGAGCGATCAAGTCA
Suggested primer 2:GGACGAAGCCATACTGGTGT