| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250425 |
| Chromosome: | chromosome 14 |
| Location: | 1680194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619250 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGATGGCGAAGCCGTTGCACCGAGCAG |
| Internal bar code: | CCCGGGGACAGATACGGCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 86.6844 |
| LEAP-Seq n confirming: | 651 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAATAAGGCGGGAGAAC |
| Suggested primer 2: | AGCAGGCGTGTATGTAGGCT |