Insertion junction: LMJ.RY0402.250432_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.250432
Chromosome:chromosome 2
Location:3878687
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g096000 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATACACAAACCCCCTCCGTCACCCAGGG
Internal bar code:CGGGCTCGTGTCACGTCTCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:655
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:413
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACATTGACCGTTTCCCT
Suggested primer 2:AGCCAGAAGACTGCGATGTT