| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250434 |
| Chromosome: | chromosome 10 |
| Location: | 5895994 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461950 | PBE1 | 20S proteasome beta subunit E, type beta 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAACCAACGGCTCCCTAAGCACTGCGC |
| Internal bar code: | GGACGGCACAAATCCGTCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 86.8322 |
| LEAP-Seq n confirming: | 1154 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGCAGGAATCGCTTTGC |
| Suggested primer 2: | CATGATGTTGGCAGTTGGAC |