Insertion junction: LMJ.RY0402.250464_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.250464
Chromosome:chromosome 9
Location:1245297
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g399550 (1 of 1) PTHR10857//PTHR10857:SF11 - COPINE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGACCCCGTCTCCCGCAGCCAAACACT
Internal bar code:GGAGGACGAATAGTTGGGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:512
LEAP-Seq percent confirming:94.9153
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AATGGGAAGTGCATAAACGC
Suggested primer 2:CGGCTCCTTCACTTGACTTC