| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250466 |
| Chromosome: | chromosome 10 |
| Location: | 2075586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433150 | (1 of 1) PF01344//PF13415 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGGGATGCCGGGTGTGGGGTGTGGGG |
| Internal bar code: | GGGGGGTCTTCGACAGTGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGACCGCTGCAAACTCT |
| Suggested primer 2: | ACGGAGCTGTATAGGAGCGA |