| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250492 |
| Chromosome: | chromosome_10 |
| Location: | 2305770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g434650 | CTR3 | Copper transport accessory protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCGCCAGCCGCGTGCCGCAGTGCAGCGCG |
| Internal bar code: | GGGACGGGCAGGTCGCAGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCACGGTGTCTGCCTAGT |
| Suggested primer 2: | CCGCACAGTATCCACATCAC |