| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250529 |
| Chromosome: | chromosome 4 |
| Location: | 163423 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216950 | KAS3 | Putative 3-ketoacyl-ACP-synthase (FAS complex); (1 of 1) 2.3.1.180 - Beta-ketoacyl-[acyl-carrier-protein] synthase III / KASIII | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGTCGCAATTAATTAGTTATCTAGTG |
| Internal bar code: | CCTGGCTCATCCGCCCTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 98.3201 |
| LEAP-Seq n confirming: | 1112 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCGCTCCAAGCATAAC |
| Suggested primer 2: | AGCATCTTGTCGATCAGCCT |