| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250540 |
| Chromosome: | chromosome 16 |
| Location: | 2935783 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664050 | (1 of 1) PF02893//PF16016 - GRAM domain (GRAM) // Domain of unknown function (DUF4782) (DUF4782) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCACCTGGCGCGCTGTAACGCCTAGGC |
| Internal bar code: | TCGGGTTGCATAGATTCCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 85.3821 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGGCCACATACGCATAC |
| Suggested primer 2: | TTAGGGCCCTGTGAGAGCTA |