Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.250543 |
Chromosome: | chromosome_3 |
Location: | 4894777 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g179500 | PHX8 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTCGTGCCGCCACCACACCATCTTGGTCT |
Internal bar code: | GGTTTCGGGCTGGGCCGAATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 117 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 104 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTGAAGTAATCAGCGGCA |
Suggested primer 2: | AGGCTTAGGCTTGGCTTAGG |