Insertion junction: LMJ.RY0402.250562_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.250562
Chromosome:chromosome_6
Location:1736058
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre06.g261750 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AATGCCACCAGTAACATCAGCGCAGGTGGC
Internal bar code:GGCAAGATAGCGCGTCTGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:172
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCCAAGCTGACCAAGAT
Suggested primer 2:GGTCAGGCATCAGGTACGTT