| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250608 |
| Chromosome: | chromosome 11 |
| Location: | 3045893 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478800 | (1 of 1) PTHR31027//PTHR31027:SF2 - FAMILY NOT NAMED // PROTEIN F18H3.4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTACTTCATTTCTGCGCTACGGCGCTG |
| Internal bar code: | TCTACTAGCCACGGGCTCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 98.8038 |
| LEAP-Seq n confirming: | 413 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGAGCAAGAAGGACAAG |
| Suggested primer 2: | ATGGCTGAAAATTTTGGTCG |