| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250633 |
| Chromosome: | chromosome 11 |
| Location: | 153194 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467544 | IDI1 | Isopentenyl-diphosphate delta-isomerase; (1 of 1) K01823 - isopentenyl-diphosphate delta-isomerase (idi, IDI) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTACTTCGATCACGCTAGCCTGGACAG |
| Internal bar code: | GTACAGATCAGACTAGAGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 99.2537 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTACTACCCCACGAGCG |
| Suggested primer 2: | TTCGTTGGAGCTCTCCTCAT |