| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250677 |
| Chromosome: | chromosome 1 |
| Location: | 3501720 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022350 | HEL3 | DEAH box ATP-dependent RNA helicase; (1 of 1) K14805 - ATP-dependent RNA helicase DDX24/MAK5 [EC:3.6.4.13] (DDX24, MAK5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAACAGCGGCAGCGTGAGCGTAGCGCTG |
| Internal bar code: | GCAGGGTTACTAGACTCGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 97.3568 |
| LEAP-Seq n confirming: | 442 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATGAGCTGCGCAGTGTG |
| Suggested primer 2: | GTGGATGGAGGAAACAGGAA |