| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250681 |
| Chromosome: | chromosome 12 |
| Location: | 7708199 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554050 | (1 of 1) IPR022348 - G protein-coupled receptor 162 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAAGTTGCGGTAAACAGATTCGTCACAA |
| Internal bar code: | CGAATTACTCAGGGAAATAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.6571 |
| LEAP-Seq n confirming: | 4360 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCAGCCACAGTCTGTATG |
| Suggested primer 2: | TTAGCACCAAGCACGCATAC |