Insertion junction: LMJ.RY0402.250704_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.250704
Chromosome:chromosome 12
Location:1097780
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g490700 MIN1 (1 of 1) IPR000008//IPR018392 - C2 domain // LysM domain; Mini-eyespot protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCTTTCCTCCCTCGCCATGTGTCTCTAT
Internal bar code:GTTTTGTCTGCGGGACATCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:569
LEAP-Seq percent confirming:99.816
LEAP-Seq n confirming:4340
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCCGAGTCGGATTACCT
Suggested primer 2:CATGCATGTTGGTGTGATGA